Below you have Phycocyanin molecule. Identify (circle the functional groups on the molecule.
science
Description
Instruction
for candidates
Answer all questions by inserting your answers in this pro-forma.
Show all steps of your calculations and show all units! You can handwrite your answers and scan them in if you prefer, but they
must be readable. Your completed assignment should be submitted as a
Word document via Turnitin (which is embedded on Moodle). Please insert your
names on the Header, before submitting the document. Total marks are out of 80.
1. Below you have Phycocyanin molecule. Identify (circle
the functional groups on the molecule.
You can do this in word by using insert/shape/[choose circle],[choose no
fill colour]) and name the functional groups present in the molecule.
[20
marks]
2. a.
Calculate the Mr of Phycocyanin molecule.
b. Use the data given below to convert the
concentration from mM to mg/ml and then
present your data in a table.
0 mM = 0.004 Abs
1.2 mM = 0.230 Abs
2.4 mM = 0.477 Abs
3.1 mM = 0.710 Abs
3.8 mM = 0.950 Abs
c. Using Excel,
plot a calibration curve for Phycocyanin using four standards and a blank. Plot
the calibration curve in concentration units of mg/ml.
[Insert Graph Here] Make sure they are properly
labelled and formatted well.
[40
marks]
b. Explain if the trend on the graph follows or does
not follow Beer’s Law
[10 marks]
3. n unknown sample of Phycocyanin was analysed and
the absorbance reading given was 0.324. Use the calibration curve you have
drawn to find out the amount of Phycocyanin in the sample and present your
answer into mM.
