CRISPR-Cas9 gene editing has come to the forefront of molecular genetics as a method to introduce mutations into any particular gene of interest to a researcher. One issue with CRISPR-Cas9, however, is that it is not possible to easily turn off the editing system. As a budding chemical biologist, you decide you are going to develop a chemical screen to identify compounds that inhibit CRISPR-Cas9 gene editing. How would you set up such a compound screen? Assume that CRISPR-Cas9 can function in all model biological systems, that is plants, animals, bacteria and yeast. Hint: This answer requires that you develop an assay in which you can easily monitor the inhibition of the CRISPR-Cas9 system. (10 marks)
In class, you were introduced to YLG, a fluorogenic probe that marks the activity of Striga ShHTL receptors involved in the perception of the plant hormone strigolactone (SL). Although YLG shows that ShHTL receptors have a complex pattern of activity during Striga germination, this probe does not tell us anything about the function of these SL receptors. Can you suggest two experiments using chemical genomics approaches that might be useful in understanding the function of any of the ShHTL receptors. Remember Striga is a poor genetic model. You cannot screen for mutants and you cannot make transgenic plants (10 marks)
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